Journal: Molecular Vision

Article Title: Calcium-binding S100 protein expression in pterygium

doi:

Figure Lengend Snippet: Primary antibodies used in the experiment.

Article Snippet: Mouse monoclonal antibodies against S100A4, S100A6, S100A9, and S100A11 were purchased from Abnova (Taipei, Taiwan), and mouse monoclonal antibody against S100A8 from Acris Antibodies (Hiddenhausen, Germany) was used.

Techniques:

Journal: Molecular Vision

Article Title: Calcium-binding S100 protein expression in pterygium

doi:

Figure Lengend Snippet: Primers used in real-time PCR.

Article Snippet: Mouse monoclonal antibodies against S100A4, S100A6, S100A9, and S100A11 were purchased from Abnova (Taipei, Taiwan), and mouse monoclonal antibody against S100A8 from Acris Antibodies (Hiddenhausen, Germany) was used.

Techniques: Sequencing

Journal: Molecular Vision

Article Title: Calcium-binding S100 protein expression in pterygium

doi:

Figure Lengend Snippet: Immunofluorescent staining of S100 proteins in human conjunctival and pterygial epithelia. Primary antibodies against S100A4 ( C and D ), S100A6 ( E and F ), S100A8 ( G and H ), S100A9 ( I and J ), and S100A11 ( K and L ) were used. Negative controls were shown in A and B . Images of uninvolved conjunctiva ( A , C , E , G , I , and K ) and pterygium epithelium ( B , D , F , H , J , and L ) were shown. The nuclei were stained with DAPI present in the mounting medium. A dashed line indicates the location of the basement membrane. All images were taken at 400X magnification.

Article Snippet: Mouse monoclonal antibodies against S100A4, S100A6, S100A9, and S100A11 were purchased from Abnova (Taipei, Taiwan), and mouse monoclonal antibody against S100A8 from Acris Antibodies (Hiddenhausen, Germany) was used.

Techniques: Staining, Membrane

Journal: Molecular Vision

Article Title: Calcium-binding S100 protein expression in pterygium

doi:

Figure Lengend Snippet: Western blot analysis of S100 proteins expressed in human conjunctiva and pterygium tissue. Uninvolved conjunctiva (Lane 1) and pterygium (Lane 2) tissues were lysed in RIPA buffer and 60 μg of total protein was loaded in each lane of SDS-PAGE and transferred to nitrocellulose membrane and probed with antibodies against various S100 proteins as well as β-actin (loading control). Bands of 11 kDa corresponding to S100A4, S100A6, and S100A11 were detected. Bands corresponding to the molecular weight of 8 kDa and 13 kDa confirmed the presence of S100A8 and S100A9, respectively.

Article Snippet: Mouse monoclonal antibodies against S100A4, S100A6, S100A9, and S100A11 were purchased from Abnova (Taipei, Taiwan), and mouse monoclonal antibody against S100A8 from Acris Antibodies (Hiddenhausen, Germany) was used.

Techniques: Western Blot, SDS Page, Membrane, Control, Molecular Weight

Journal: Molecular Vision

Article Title: Calcium-binding S100 protein expression in pterygium

doi:

Figure Lengend Snippet: Fold difference of S100A4 , S100A6 , S100A8 , S100A9 , and S100A11 gene transcripts in pterygium tissue relative to conjunctiva tissue. Total RNA was extracted from uninvolved conjunctiva and pterygium tissue after mechanical homogenization. Reverse transcription for 0.5 μg of total RNA. Glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) was used as the internal control. ΔC t was calculated by subtracting the C t of GAPDH from the C t of the targeted gene. The fold change was determined by the ΔΔC t method (see text). The uninvolved conjunctiva sample was used as a reference for each of the tested S100 transcripts in the pterygium sample. Height of the bars represents mean value and error bars represent standard deviation. An asterisk indicates that p is less than 0.05 (p<0.05).

Article Snippet: Mouse monoclonal antibodies against S100A4, S100A6, S100A9, and S100A11 were purchased from Abnova (Taipei, Taiwan), and mouse monoclonal antibody against S100A8 from Acris Antibodies (Hiddenhausen, Germany) was used.

Techniques: Homogenization, Reverse Transcription, Control, Standard Deviation